qPCR Data Analysis


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Documentation for package ‘rtpcr’ version 2.0.5

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ANOVA_DCt Relative expression (Delta Ct method) analysis using ANOVA
ANOVA_DDCt Relative expression (Delta Delta Ct method) analysis using ANOVA and ANCOVA
efficiency Slope, R2 and Efficiency (E) statistics and standard curves
Means_DDCt Delta Delta C_T analysis analysis using a model
meanTech Calculating mean of technical replicates
multiplot Multiple plot function
plotOneFactor Bar plot of gene expression from an expression table of a single-factor experiment data
plotThreeFactor Bar plot of gene expression from an expression table of a three-factorial experiment data
plotTwoFactor Bar plot of gene expression from an expression table of a two-factorial experiment data
REPEATED_DDCt Fold change (Delta Delta C_T method) analysis of repeated measure qPCR data
TTEST_DDCt Expression (Delta Delta C_T method) analysis of target genes using t-test